Components

Influenza A Reagent - (Catalog No. 5017). One 2 mL dropper vial containing FITC labeled influenza A antibody, protein stabilizer, Evans Blue and 0.1% sodium azide (preservative).Influenza B Reagent- (Catalog No. 5018). One 2 mL dropper vial containing FITC labeled influenza B antibody, protein stabilizer, Evans blue and 0.1% sodium azide (preservative).Influenza A/B Control Slides - (Catalog No. 5010). Two slides containing one well of influenza A infected cells, one well of influenza B infected cells, and two wells of uninfected cells.Phosphate Buffered Saline - (Catalog No. 5087). One packet of phosphate buffered saline (PBS) salts.Tween 20 / Sodium Azide Solution (100 X) - (Catalog No. 5037). One 10 mL vial containing Tween 20, and sodium azide concentrate.Mounting Fluid - (Catalog No. 5013). One 10 mL dropper vial containing Tris-buffer and glycerin, a fluorescence enhancer, and sodium azide (preservative).

Disclaimer

CE MarkFor in vitro Diagnostic Use

General description

Diagnostics Kit

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage and Stability

When stored at 2-8° C, the Light Diagnostics Influenza A and B DFA Kit is stable up to the expiration date printed on the label. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the kit expiration date. During incubation, slides should be protected from light and kept in a humid chamber at the recommended temperature. A marked decrease in fluorescence may indicate conjugate deterioration. A positive

control should be tested with each specimen to ensure proper functioning of these reagents and proper staining procedure. If after appropriate analysis there is a decrease in staining intensity, discontinue use of the reagents.

Warnings and Precautions:

⋅ Sodium azide (NaN3) used as a preservative in the conjugates, PBS/Tween and Mounting Fluid is toxic if ingested. Sodium azide may react with lead or copper plumbing to form highly explosive metal azides (17, 18). Upon disposal, flush with large volumes of water to prevent build-up in plumbing.

⋅ Handle all specimens and materials coming in contact with them as potentially infectious and dispose of with proper precautions. Decontaminate with 0.05% sodium hypochlorite (bleach).

⋅ Acetone is extremely flammable and harmful if swallowed or inhaled. Keep away from heat, sparks or flame. Avoid breathing vapor. Use adequate ventilation.

⋅ Do not mouth-pipette reagents.

⋅ Avoid contact with Evans Blue (present in conjugates), as it is a potential carcinogen. If skin contact occurs, flush with large volumes of water.

⋅ Mounting Fluid contains a fluorescence enhancer that may be destructive to mucous membranes. Avoid direct skin or mucous membrane contact. If contact occurs, flush with large volumes of water.

⋅ Pooling or alteration of any reagent may cause erroneous results.

⋅ Do not use reagents beyond expiration date.

⋅ Do not substitute reagents from other manufacturers.

⋅ Incubation times or temperatures other than those specified may give erroneous results. Any such change must be validated by the user.

⋅ Do not allow shell vials or slides to dry at any time during the staining procedure.

⋅ When staining multiple samples on a slide, avoid cross contamination between samples.